5 Tips about attune flow cytometer You Can Use Today
5 Tips about attune flow cytometer You Can Use Today
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Data-enriched characterization of mobile populations. Morphological info from photos can increase to the richness of flow cytometry info. For instance, the figure displays an if not regular apoptosis assay utilizing Annexin V and PI, introducing cell imaging to characterize cells in Each individual population to expose morphologically distinct functions. These insights couldn't are obtained from multiplex staining by itself.
As samples are acquired on the Attune CytPix Flow Cytometer, the higher-speed brightfield digicam captures and retailers illustrations or photos of detected events in a charge around 6,000 images per 2nd, according to the flow fee and graphic dimensions. For bigger versatility, Attune Cytometric Computer software allows you to modify the picture capture frequency as necessary and select a particular gate for graphic capture, if essential.
Mix acoustic focusing technologies with superior-parameter analysis for adaptable spectral unmixing and traditional workflows.
Explore new insights into your experimental info by visualizing populations utilizing put together fluorescence and morphology info. Our program interface wizards enable downsampling, concatenation (merged data files) and dimensionality instruments which are constructed in the software for an intuitive, streamlined data Evaluation knowledge.
"An image isn't rather worthy of a thousand dots, but it surely truly helps to see visuals. It is excellent to discover images that correlate with staining. Such as, AnnexinV-good cells that are morphologically various than nutritious cells, or CD14+ monocytes that happen to be more substantial plus much more textured than CD3+/CD4+/CD14- lymphocytes.
Right here, a seasoned person has gated singlets confidently. Immediately after evaluating the handbook singlet gate, the CytPix image-derived parameter ParticleCount reveals this gate contains a lot more than four% aggregates.
Pair your Attune flow cytometer using an autosampler for elevated workflow effectiveness, and increase robotic integration for optimum throughput.
Regular graphic good quality even at significant throughput Acoustic concentrating plus a higher-velocity camera combine to picture these T cells regularly at very low or substantial flow premiums. Effortlessly change aim and digicam settings to fulfill experimental necessities.
The Attune NxT cytometer would be the dependable workhorse instrument your lab can count on for dependable and dependable performance. The Attune CytPix design encompasses a superior-speed brightfield camera and AI-driven image Examination computer software, with the latest upgrades including device learning resources and an intuitive consumer interface, enabling Improved facts precision, precision, and more quickly insights into sample biology.
Users can look at celebration pictures in authentic-time to confirm sample good quality, optimize protocols, and document the morphology of every mobile inhabitants identified for Examination. Imaging capture also Positive aspects from acoustic concentrating, which helps you to placement the cells to ensure a sharp, centered picture is attained.
AI-pushed automatic image Investigation Along with the Attune Cytometric Computer software translates event capabilities into distinct morphology parameters which can be coupled with common fluorescence and scatter parameters to expose highly reproducible populace alterations and special subsets.
We Formerly shown the power of imaging Automobile-T/Ramos mobile interactions. Permit’s evaluate just the population of greatest fascination, the double constructive activities, to learn more. We are able to now use extended morphological parameters (in this article, circularity and skewness of intensity) to even further study the characteristics of those populations and refine gating on these situations, expanding details robustness.
Leverage the strength of Innovative technological innovation and innovation to speed up your discoveries. Have faith in while in the Attune Flow Cytometer spouse and children to deliver the trustworthiness, pace, and accuracy that set your work aside.
Adaptable payment system from Agilent Money Solutions let you receive the important instrumentation you will need for rapid venture, or to expand your lab.
Gating on these extended parameters permits you to swiftly and accurately establish populations of curiosity and ensure gating method with little if any guide overview. The Attune software program provides entry to point out-of-the-art machine Understanding Investigation equipment in an expertly made consumer interface, which makes it exceptionally simple to operate.
As the cell line had Earlier been analyzed within the Attune CytPix Flow Cytometer, the scientists went back to the photographs and ended up capable of doc the microbial an infection no less than five days before.
Agilent Value Promise ensures you not less than check here 10 years of instrument use from your date you purchase. Normally, We are going to credit score you Along with the residual worth of the method toward an upgraded product.
In this article, we show that by gating approaches utilizing picture-based quantitative parameters can distinguish interacting cells from coincident gatherings to far more precisely examine interacting cells.